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Polyscience，Inc，成立于1961年，起家于化學但超越化學領域的美國制造商，總部位于賓夕法尼亞州沃靈頓，公司業(yè)務主要涵蓋三大塊：1.實驗用產品：a）成千上萬種具品牌特色的化合物和試劑，應用于實驗室研究，組織學，電鏡和解剖病理學以及其他診斷和生命科學研究；b）種類極其寬廣的單體和聚合體，滿足各方研究領域；c）有機/無機化合物，生物可降解材料，磁珠，熒光素，染料和特定抗體，以及常規(guī)蛋白包被顆粒（直徑范圍40nm~10mm）的首要制造工廠；d）頂端多聚化合物的供貨中心，滿足各種高性能工業(yè)領域需求，包括替代能源，汽車電子，國防&電子，便攜式通信和計算機，醫(yī)療器械組裝。2. 定制合成；3.cGMP生產服務。：1（800）523-2575；（215）343-6484。
Brand Story：
Me，Polyscience，Inc，Chemistry beyond the ordinary
Me，Polyscience，Inc，Headquartered at Warrington，PA，Call vial 1（800）523-2575
Me，Polyscience，Inc，Manufacturing hundreds of specialty chemicals and reagents for laboratory research, Histology, Light Microscopy and Anatomic Pathology as well as other clinical and life science applications.
Me，Polyscience，Inc，Stocking a wide portfolio of monomers and polymers for research applications.
Me，Polyscience，Inc，Developed to be the primary source for organic, inorganic, biodegradable, magnetic, fluorescent, dyed and specific antibody and general protein coated particles in diameters spanning the range of 40nm to 10mm.
Me，Polyscience，Inc，Offering innovative polymer products to meet the high performance requirements of a variety of industries including alternative energy, automotive electronics, defense & aerospace electronics, portable communications & computing, medical device assembly, and more.
——Written/Edited by V. Shallan
Special Products：
你會喜歡的第二代聚合物轉染試劑
Polyethylenimine，Linear，Mw 25，000 線性化聚乙烯亞胺（PEI 25，000）
基本描述：
線性化聚乙烯亞胺PEI 25，000，一種高電荷陽離子聚合物，非常容易結合于高電荷陰離子基質。工業(yè)應用上，線性化PEI可改善負電荷染料的物理外觀，以調整染料的化學特性和增強染料與固相基質的黏附能力，常用作黏附增強劑，作用同多聚賴氨酸（poly-lysine）；科研應用上，線性化PEI能與DNA或其他負電荷生物大分子簡單結合，正是這一特性，使得成為一種非常行之有效的載體運輸介質（Vector carrier），即轉染試劑。
化學性質：
CAS NO. | 9002-98-6, 26913-06-4 |
Formula | (CH2CH2NH)n |
Molecular Weight | 25,000 |
Melting Point | 73-75º |
Solubility | Soluble In: hot water, cold water at low pH, methanol, and ethanol. Insoluble In: benzene, ethyl ether and acetone |
Appearance | White to yellow solid |
訂購信息：【*，現(xiàn)貨供應】
品牌 | 貨號 | 名稱 | 規(guī)格 | *（元） |
Polysciences | 23966-2 | 線性聚乙烯亞胺（MW 25，000） | 2g | 2684 |
另，提供更高轉化效率的PEI Max 40，000，自由基形式分子量相當于PEI 25，000，研究證實其體外質粒DNA轉染效率提高達21倍。 | ||||
Polysciences | 24765-2 | 線性聚乙烯亞胺鹽酸鹽Max，（MW 40，000） (Equivalent to Mw 25,000 in Free Base Form) | 2g | 3184 |
使用方法【來源于：CSH protocols，僅作交流學習】 ：
1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.
2. Add 500 mg of PEI to the beaker with stirring.
3. Add concentrated HCl drop-wise to the solution to pH <2.0.
4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.
Approximay 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.
5. Add concentrated NaOH drop-wise to the solution to pH 7.0.
Approximay 500 μL of 10 M NaOH will be required to neutralize the PEI solution.
6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.
7. Filter-sterilize the solution through a 0.22-μm membrane.
8. Store aliquots of the desired volume at -20°C.
應用文獻【基因轉染用途】 ：
Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.
Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pin (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98.
Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.
——Written/Edited by V. Shallan【版權歸MKBio懋康所有】
更的PEI聚乙烯亞胺聚合物轉染試劑
Polyethylenimine HCl MAX, Linear, Mw 40,000
線性化聚乙烯亞胺鹽酸鹽 MAX（PEI 40，000）
基本描述：
Polyethylenimine HCl MAX, Linear, Mw 40,000（PEI 40，000）是熱賣產品線性化聚乙烯亞胺PEI25,000的升級產品，不同之處在于1）*水解（去乙?；?；2）PEI25，000的鹽酸鹽形式，具更高的水溶特性；3）含更長連續(xù)性乙烯亞胺（ethyleneimine）片段，其質子化氮水平比PEI25，000提高11%以上。本品經(jīng)堿中和后，可轉化為線性化自由胺（PEI），此時分子量正相當于25,000。
Thomas M，et al（2005）研究數(shù)據(jù)表明，去乙?；?/span>PEI 40,000（Polyethylenimine MAX 40,000）體外質粒DNA轉染效率提高21倍，小鼠體內靶向效率達到10,000倍，隨之肺部特異療效顯示增強1500倍。
化學性質：
CAS NO. | 9002-98-6, 26913-06-4 |
Molecular Weight | 40,000（~22,000 free base） |
Solubility | Soluble In: Cold and room temperature water Insoluble In: Common organic solvents (ethanol, acetone, tetrahydrofuran) |
Appearance | White to off-white free flowing solid |
訂購信息：【*，現(xiàn)貨供應】
品牌 | 貨號 | 名稱 | 規(guī)格 | *（元） |
Polysciences | 24765-2 | 線性聚乙烯亞胺鹽酸鹽Max，（MW 40，000） (Equivalent to Mw 25,000 in Free Base Form) | 2g | 3184 |
另，提供基本型PEI 25，000。 | ||||
Polysciences | 23966-2 | 線性聚乙烯亞胺（MW 25，000） | 2g | 2684 |
應用文獻【基因轉染用途】 ：
Mann JF, McKay PF, Arokiasamy S, Pa RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
——Written/Edited by V. Shallan【版權歸MKBio懋康所有】